ISOTHERMAL NUCLEIC ACID AMPLIFICATION

An isothermal process for amplifying a double-stranded nucleic acid target molecule that relies on upstream and downstream primers and an oligonucleotide, wherein at least a portion of the oligonucleotide is complementary to a portion of the target sequence intervening the upstream and downstream pr...

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Bibliographic Details
Main Author Hoser, Mark Jay
Format Patent
LanguageCroatian
English
Published 17.11.2017
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:An isothermal process for amplifying a double-stranded nucleic acid target molecule that relies on upstream and downstream primers and an oligonucleotide, wherein at least a portion of the oligonucleotide is complementary to a portion of the target sequence intervening the upstream and downstream primers, wherein the upstream and downstream primers do not amplify the target molecule independently of the oligonucleotide and wherein the oligonucleotide facilitates the separation of the double-stranded nucleic acid target molecule thereby allowing the primers access to bind to complementary single-stranded DNA on the target molecule.
Bibliography:Application Number: HR2017P001286T