Enzymes with RuvC domains

An engineered nuclease system, comprising: (a) an endonuclease comprising a RuvC_III domain and an HNH domain, wherein said endonuclease is a class 2, type II Cas endonuclease; and (b) an engineered guide ribonucleic acid structure configured to form a complex with said endonuclease comprising: (i)...

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Bibliographic Details
Main Authors Daniela S.A. Goltsman, Christopher Brown, Lisa Alexander, Rose Kantor, Cristina Butterfield, Audra Devoto, Brian Thomas, Jason Liu
Format Patent
LanguageEnglish
Published 01.05.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:An engineered nuclease system, comprising: (a) an endonuclease comprising a RuvC_III domain and an HNH domain, wherein said endonuclease is a class 2, type II Cas endonuclease; and (b) an engineered guide ribonucleic acid structure configured to form a complex with said endonuclease comprising: (i) a guide ribonucleic acid sequence configured to hybridize to a target deoxyribonucleic acid sequence; and (ii) a tracr ribonucleic acid sequence configured to bind to said endonuclease, wherein said endonuclease comprises a sequence having at least 80% specificity to SEQ ID NO. 423. The endonuclease can comprise a RuvC_III domain with at least 75% sequence identity to SEQ ID NOs. 1827-3637. The endonuclease can be configured to be selective for a protospacer adjacent motif (PAM) sequence selected from the group consisting of SEQ ID Nos: 5512-5537.
Bibliography:Application Number: GB202219441