Modified pipette tips for chromatin immunoprecipitation assay

A pipette tip (20) containing a rigid porous matrix (22) on which a ligand is immobilized, the ligand being capable of binding to a protein associated with chromatin; the rigid porous matrix being positioned within the pipette tip such that, in use, chromatin in a liquid sample passing through the p...

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Bibliographic Details
Main Authors David Richard Cowieson, Lewis Francis, Samantha Jane Hutchinson
Format Patent
LanguageEnglish
Published 04.10.2017
Subjects
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY
CHEMISTRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
SEPARATION
TESTING
THEIR RELEVANT APPARATUS
TRANSPORTING
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Summary:A pipette tip (20) containing a rigid porous matrix (22) on which a ligand is immobilized, the ligand being capable of binding to a protein associated with chromatin; the rigid porous matrix being positioned within the pipette tip such that, in use, chromatin in a liquid sample passing through the pipette tip is retained by the rigid porous matrix is described. A method of isolating chromatin from the liquid sample using the pipette tip and the use of the pipette tip in a chromatin immunoprecipitation assay are also described.
Bibliography:Application Number: GB20140021197