Yeast microorganisms genetically engineered to improve isobutanol biosynthesis
A recombinant yeast the expresses either an endogenous or overexpressed endogenous gene of an enzyme that catalyses at least a step in the conversion of pyruvate to isobutanol, and is further engineered to reduce or eliminated an endogenous aldehyde dehydrogenase (ALDH or ALD6) that catalyses the co...
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Main Authors | , , , , , , , , , , , |
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Format | Patent |
Language | English |
Published |
16.10.2013
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Subjects | |
Online Access | Get full text |
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Summary: | A recombinant yeast the expresses either an endogenous or overexpressed endogenous gene of an enzyme that catalyses at least a step in the conversion of pyruvate to isobutanol, and is further engineered to reduce or eliminated an endogenous aldehyde dehydrogenase (ALDH or ALD6) that catalyses the conversion of isobutyraldehyde to isobutyrate and also an endogenous pyruvate decarboxylase (PDC) that catalyses the conversion of pyruvate to acetaldehyde. The yeast may further have a glycerol-3-phosphate dehydrogenase (GPD) deletion or mutation. The recombinant microorganisms may be microorganisms of the Saccharomyces Glade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms, including Sacchormyces, Kluyveromyces, Pichia, Hansenula, Issatchenkia and Candida. Methods of isobutanol production are also claimed. |
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Bibliography: | Application Number: GB20120020508 |