RNA isolation method

• Main Authors: ARNE DEGGERDAL, ANDREW CAMPBELL, VERONICA BOGER
• Format: Patent
• Language: English
• Published: 23.06.2010
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A method for isolating RNA from a biological sample, which comprises: (i) treating the sample with a lysis solution comprising a detergent at a pH of greater than 6 to form a lysate comprising an RNA-containing liquid phase; (ii) contacting the lysate with a first solid phase bearing a positive charge in the presence of a salt so as to precipitate DNA and protein from the RNA- containing liquid phase onto the first solid phase; (iii) separating the RNA-containing liquid phase from the solid phase; (iv) contacting the RNA-containing liquid phase with a non-ionic solid phase in the presence of a lower alcohol comprising ethanol or propanol to precipitate RNA onto the non-ionic solid phase to form an RNA-containing solid phase; (v) separating the RNA-containing solid phase from the liquid phase; and (vi) isolating RNA therefrom.