No wash immunoassay

A device for the assay has a base member bearing a test zone and at least one immobilized immunological component disposed in said zone in indicia-forming configuration. The component is operable to bind enzyme-labeled antibody through a target analyte to form a sandwich complex on said zone which c...

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Bibliographic Details
Main Authors CHIYOUNG OK, BYUNGWOO YOUN, WALTER J KANG, DONG JOON LEE, JEMO KANG, JOHN A COLANDUONI
Format Patent
LanguageEnglish
Published 09.02.1994
Edition5
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:A device for the assay has a base member bearing a test zone and at least one immobilized immunological component disposed in said zone in indicia-forming configuration. The component is operable to bind enzyme-labeled antibody through a target analyte to form a sandwich complex on said zone which complex produces upon contact with a substrate for said enzyme a visually discernible difference between said configuration and said test zone. Immobilized component is e.g. an antibody, antigen or hapten. Enzyme can be horseradish peroxidase, urease, glucose oxidise or a dehydrogenase. Base members may be of glass or plastic. Target analyte may be an antibody, antigen or hapten. Indicia configuration is e.g. a bar, dot, number, letter or plus sign.
Bibliography:Application Number: GB19910000491