Process for the preparation of heparin salts

• Main Authors: JANOS ILLES, PAL GERE, GYORGY KEREY, BELA STEFKO, ISTVAN ZAMBO, ERZESBET NESZMELYI
• Format: Patent
• Language: English
• Published: 17.12.1986
• Edition: 4
• Subjects: CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; POLYSACCHARIDES; THEIR PREPARATION OR CHEMICAL WORKING-UP

Heparin salts of pharmacopoeial purity, preferably calcium heparinate are prepared by treatment of an aqueous solution of a heparinate of pharmacopoeial quality or a pyrogen-free aqueous heparinate solution optionally containing heavy metal salts and inactive heparinoid fractions with a quaternary ammonium halide, separating the precipitated heparin-quaternary amine complex formed and washing it with pyrogen-free, deionized water, and dissolving the purified heparin-quaternary amine complex i) in an aqueous solution of a salt containing the cation of the heparinate to be prepared, or ii) in a water-immiscible organic solvent, and adding to the solution obtained an aqueous solution of a salt containing the cation of the heparinate to be prepared, or iii) in an organic solvent immiscible or restrictedly miscible with water, and adding to the solution obtained an aqueous solution of a salt containing the cation of the heparinate to be prepared, allowing the two phases to separate and separating them from each other, and if desired, adding to the organic phase obtained in step iii) an aqueous solution of a salt containing the cation of the heparinate to be prepared, allowing the phases to separate, separating them from each other and combining the aqueous phases.