Method and reagents for the use of beta -D-galactosidase as a tracer in immunocytochemistry

beta -D-galactosidase is used as tracer in an immunoenzyme cytochemical method for the identification of antigens in tissues. The enzyme tracer may be linked to the antigen via a specific binder e.g. an antibody specific to the antigen and the antigen can then be revealed by action of the enzyme tra...

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Bibliographic Details
Main Authors GIORGIO CHIEREGATTI, ENZO MURADOR
Format Patent
LanguageEnglish
Published 10.08.1983
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:beta -D-galactosidase is used as tracer in an immunoenzyme cytochemical method for the identification of antigens in tissues. The enzyme tracer may be linked to the antigen via a specific binder e.g. an antibody specific to the antigen and the antigen can then be revealed by action of the enzyme tracer on a chromogenic substrate which converts to an insoluble coloured product. A reagent kit for use in the method comprises (1) an antibody specific to the antigen, the tracer enzyme which can be in the form of a conjugate and (2) the chromogenic substrate. A universal reagent comprises the chromogenic substrate and a beta -D-galactosidase conjugated anti-antibody. The use of beta -D- galactosidase has many advantages over the use of the conventional peroxidases.
Bibliography:Application Number: GB19830001305