Using inhibitors of protein FKBP65 for treating cancer and other diseases where angiogenesis is involved, also new isoforms of FKBP65, related nucleic acids and diagnostic uses

• Main Authors: SCHWEIGHOFFER FABIEN, VALENTIN EMMANUEL, PETIT SILVERE
• Format: Patent
• Language: English; French
• Published: 25.06.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

Use of an inhibitor (A) of protein FKBP65 to prepare a composition for treating cancer; for inhibiting angiogenesis, and for treating diabetic retinopathy, macular degeneration, atherosclerosis, arthritis, rheumatoid polyarthritis, psoriasis and disorders of wound healing. Independent claims are also included for the following: (1) polypeptides (I) having any of 3 sequences (644, 601 and 431 amino acids (aa), reproduced) or their fragments; (2) nucleic acid (II) that encodes (I); (3) nucleic acid probes (III) that can detect the FKBP65 gene, or corresponding RNA, by selective hybridization, containing at least part of any of 4 sequences (2641 (1), 2824 (3), 2698 (5) or 2630 (7) bp, reproduced), or their fragments; (4) single-stranded primer (IV) that contains 6-50 nucleotides (nt) complementary to at least part of sequence (1), (3), (5) or (7), to their complements or their border regions; (5) method for detecting a disease, or predisposition, or for monitoring or staging, by detecting the presence, amount or distribution of FKBP65 protein, its expression, altered forms of it, or the corresponding mRNA; (6) method for selecting, screening, characterizing, optimizing or producing active compounds by determining their capacity to interact with FKBP65 (gene or protein) or to modulate its expression or activity; (7) cloning or expression vector that contains (II); and (8) recombinant cells containing the vector of (7) or (II). La présente demande concerne le domaine de la biologie et de la santé. Elle concerne plus particulièrement l'identification et la caractérisation fonctionnelle d'une nouvelle protéine impliquée dans les mécanismes de l'angiogénèse, ainsi que l'utilisation de cette protéine comme cible thérapeutique, diagnostique ou de criblage. Elle porte également sur des outils utilisables pour la mise en oeuvre de méthodes thérapeutiques, diagnostiques ou de criblage, comme notamment des sondes, anticorps, vecteurs, cellules recombinantes, etc. L'invention est particulièrement utile pour la préparation de composés pharmaceutiques dans le domaine des cancers, de l'angiogenèse, des maladies inflammatoires ou des altérations tissulaires.