New nucleic acid from Photorhabdus luminescens, useful for producing insecticidal polypeptides and insect resistant transgenic plants

• Main Authors: CHETOUANI FARID, GLASER PHILIPPE, KUNST FRANK, BOEMARE NOEL, BUCHRIESER CARMEN, FRANGEUL LIONEL, DUCHAUD ERIC, NELSEN LE ROUX CHRISTINA, RUSNIOK CHRISTOPHE, TAOURIT SEAD
• Format: Patent
• Language: English; French
• Published: 24.10.2003
• Edition: 7
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TRAPPING; VINEGAR; WINE

Isolated nucleic acid (I) sequences of 402 bp (2) and 1260 bp (4), fully defined in the specification that encode at least one polypeptide (II) active against insects, are new. Independent claims are also included for: (1) a nucleic acid sequence (Ia) that: (a) is at least 70% identical with (I); (b) hybridizes under highly stringent conditions with (I); (c) is complementary to (a) or (b); (d) is a fragment of (2) or (4), or their complements, of at least 10 bp; (e) comprises (a)-(d); or (f) is a modified form of (a)-(e), provided they encode a (II); (2) a polypeptide (II) that: (a) is encoded by (I) or (Ia), or has (a) sequence (3) or (5) of 133 and 419 amino acids (aa), respectively; or (b) has at least 80% identity with (a), or (c) is a fragment of (a) with at least 10 consecutive aa; (3) a nucleic acid (Ib) that encodes (II); (4) an expression cassette (EC) containing a promoter linked to (I), (Ia) or (Ib); (5) a cloning and/or expression vector containing EC, (I), (Ia) or (Ib); (6) a viral vector containing (I), (Ia) or (Ib) in its genome, optionally linked to an inducible or constitutive promoter; (7) host cells that contain EC; (8) producing plants that express an insecticidal toxin by introducing EC into at least one cell, then regenerating the cell to a plant; (9) plants or their parts that contain EC; (10) controlling insects by applying a composition containing (II), the cells of (7) or the vector of (5) to infected plants; (11) producing (II) by culturing cells of (7); (12) producing insect-resistant plants by introduction of (I), (Ia) or (Ib); (13) isolated nucleic acid (Ic) comprising about 60 kb from Photorhabdus luminescens, designated plBAC1A2 (CNCM I-2699), present in Escherichia coli DH10B; and (14) an isolated nucleic acid having a 60131 bp sequence (1), reproduced; and (15) strain containing the vector of (5) deposited as plbac1a2. L'invention concerne notamment des séquences nucléotidiques isolées de Photorhabdus luminescens, des toxines codées par lesdites séquences, des procédés d'obtendon de telles toxines, des compositions comprenant de telles toxines destinées à la lutte contre des insectes ravageurs des cultures ou nuisibles (car vecteurs de maladies) pour l'homme et les animaux, des plantes transformées exprimant de telles toxines.