Detecting components of microflora, useful e.g. for diagnosis of intestinal disorders, by identifying amplicons from the intergene region of a common operon

• Main Authors: MEINIEL OLIVIER, ALRIC MONIQUE, ROMOND PIERRE CHARLES, RENAUD MICHEL
• Format: Patent
• Language: English; French
• Published: 05.04.2002
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Detecting components (A) of a microflora in which at least some (A) contain a common operon by preparing genomic DNA or mRNA from the microflora, amplifying at least part of the intergene, non-coding sequence (I) present in the operon, and conserved among at least some (A), and identifying amplified (I), and thus (A). Independent claims are also included for the following: (1) a DNA chip having, on its surface, sequences (II) complementary to (I); (2) diagnostic kit for the new process comprising degenerate primers for amplification of one or more (I), and optionally the DNA chip; (3) primers for the process having any of 52 sequences fully defined in the specification; and (4) genomic microbial sequences (III) obtained by amplification using specified pairs of the primers. La présente invention concerne un procédé de détection des éléments constitutifs d'une flore de microorganismes, dont au moins une partie des éléments possède un opéron en commun, caractérisé en ce que l'on identifie les éléments de ladite flore par l'étude de la séquence intergénique dudit opéron.