DISPOSITIF AMELIORE D'IMMUNOTEST DU TYPE PLAQUETTE A PLONGER, ET TROUSSE CONTENANT CE DISPOSITIF

• Main Authors: YOUN BYUNGWOO, LEE DONG JOON, KANG WALTER J, KANG JEMO, COLANDUONI JOHN A, OK CHIYOUNG
• Format: Patent
• Language: French
• Published: 12.07.1991
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

La présente invention concerne un dispositif d'immunotest du type plaquette à plonger, dans lequel on utilise des anticorps marqués par des enzymes pour détecter des analytes dans un échantillon de fluide biologique, le résultat de l'essai apparaîssant sous forme de symboles dans la zone d'essai. Avec le dispositif de l'invention, on n'a plus besoin d'effectuer les lavages intermédiaires nécessités par les dispositifs connus d'immunotest du même type. The invention pertains to dipstick immunoassay devices. The device comprises a base member and a single, combined sample contact zone and test zone, wherein the test zone incorporates the use of symbols to detect analytes in a sample of biological fluid. A first immunological component, an anti-immunoglobulin capable of binding to an enzyme-labeled antibody, is immobilized in a control indicia portion. A second immunological component, capable of specifically binding to a target analyte which is bound to the enzyme-labeled antibody to form a sandwich complex, is immobilized in a test indicia portion. The enzyme-labeled antibody produces a visual color differential between a control indicia portion and a non-indicia portion in the test zone upon contact with a substrate. The device additionally includes a first polyol and a color differential enhancing component selected from the group consisting of an inhibitor to the enzyme and a competitive secondary substrate for the enzyme distributed throughout the non-indicia portion of the test zone.