Nebramycin antibiotic complex microbiological prodn. - by cultivation of nebramycin-resistant strains of Streptomyces tenebrarius

• Main Author: ISTVAN OTT, GABOR AMBRUS, JOZSEF GYIMESI, ILONA HARSANYI, JANOS BERDY, VALERIA SZELL, TIBOR BALOGH, LASZLO TOLGYESI, I. KADAR, LASZLO NAGY, JANOS ERDEY, E. SZUCS, ISTVAN KASZAB, FERENC FABIAN, KALMAN POLYA, LAJOS KUN, JANOS VIG, JENO SZILAGYI ET CSABA NAGY
• Format: Patent
• Language: English; French
• Published: 21.12.1979
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; SPIRITS; SUGARS; VINEGAR; WINE

In a new process for the microbioglical prodn. of the nebramycin complex, (a) a strain of Streptomyces tenebrarius which biosynthesises the nebramycin complex is cultivated in a nutrient medium contg. nebramycin 2, nebramycin 5' and/or nebramycin 6 and the individuals forming growing aerial mycelium and spores in the presence of 10-30 mg ml of these antibiotics are isolated; (b) the resulting new resistant strains are cultivated in a submerged aerobic culture in a nutrient medium contg. N- and C- sources, inorganic salts and (opt.) vegetable and/or animal fats at 30-42 degrees C (pref. 35-38 degrees C); and the nebramycin complex or nebramycin 2, nebramycin 4 and nebramycin 5' accumulating in the medium is isolated. Nebramycin 2 (apramycin) is used in the treatment of diseases of animals and plants (cf. US 3691279, 3853709, 3876767). Nebramycin 6 (tobramycin), produced by hydrolysis of nebramycin 5' (6"-O-carbamoyl-tobramycin), is a broad-spectrum antibiotic used in human medicine, esp. against polyresistant Pseudomonas strains. Use of selected nebramycin-resistant Streptomyces tenebrarius strains allows the prodn. of cultures with high nebramycin concns. thus giving improved yields. La présente invention est relative à un nouveau procédé de préparation du complexe de nébramycine. Ce procédé est caractérisé en ce que l'on cultive une souche de Streptomyces tenebrarius dans un milieu de culture qui contient de la nébramycine-2 et/ou de la nébramycine-5' et/ou de la nébramycine-6, en ce qu'on isole les individus qui forment du mycelium aérien et des spores et en ce qu'on cultive les nouvelles souches résistantes obtenues de la sorte dans un milieu de culture et à des températures appropriées. Application à la préparation du complexe de nébramycine par voie microbiologique.