Immunologisia määritysmenetelmiä ja niissä käytettävä reagenssi
A method for obtaining an actual linear standard curve in a sandwich type of immunoassay where a first antibody bound to an insoluble support and a second unbound labelled antibody complex with the antigen contained in a test sample to form an insoluble antibody:antigen:labelled antibody complex whi...
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Main Authors | , , , |
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Format | Patent |
Language | Finnish Swedish |
Published |
25.03.1994
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Edition | 5 |
Subjects | |
Online Access | Get full text |
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Summary: | A method for obtaining an actual linear standard curve in a sandwich type of immunoassay where a first antibody bound to an insoluble support and a second unbound labelled antibody complex with the antigen contained in a test sample to form an insoluble antibody:antigen:labelled antibody complex which is then detected. Unbound unlabelled first antibody and/or unbound unlabelled second antibody are added to the reaction mixture to divert excess antigen away from the desired end-product complex, thus rendering the antigen of interest the rate-limiting factor in the overall immunoreaction. This results in a pseudo first-order reaction which produces an actual linear standard curve. |
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Bibliography: | Application Number: FI19880006000 |