A method for preparing microbeads, microbeads, and a cell culture and a cell storage system comprising the microbeads

• Main Authors: Cottrell, Graeme S, Widera, Darius, Sheard, Jonathan, Eilers, Wouter, Coopman, Karen, Nuopponen, Markus, Dragosavac, Marijana, Barrett, Lisa
• Format: Patent
• Language: English; Finnish; Swedish
• Published: 14.03.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OF MACROMOLECULAR COMPOUNDS; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; PERFORMING OPERATIONS; PEST REPELLANTS OR ATTRACTANTS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; THEIR PREPARATION OR CHEMICAL WORKING-UP; THEIR RELEVANT APPARATUS; TRANSPORTING; TRAPPING

The present disclosure provides a method for preparing microbeads comprising nanofibrillar cellulose, the method comprising providing a dispersion of chemically anionically modified nanofibrillar cellulose having a number-average diameter of 200 nm or less, forming the nanofibrillar cellulose into microbeads, to obtain microbeads comprising chemically anionically modified nanofibrillar cellulose in the range of 0.2-2% by weight. The present disclosure also provides the microbeads, a cell culture, and a cell storage system. Esillä oleva kuvaus tarjoaa menetelmän nanofibrilliselluloosaa käsittävien mikrohelmien valmistamiseksi, jossa menetelmässä järjestetään saataville dispersio, jossa on kemiallisesti anionisesti muunnettua nanofibrilliselluloosaa, jolla on lukukeskimääräinen halkaisija 200 nm tai pienempi, muodostetaan nanofibrilli-selluloosasta mikrohelmiä, jolloin saadaan mikrohelmiä, jotka käsittävät 0,2-2 paino-% kemiallisesti anionisesti muunnettua nanofibrilliselluloosaa. Tässä kuvauksessa esitetään myös mikrohelmet, soluviljelmä sekä solujen säilytysjärjestelmä.