Chromatographic process

• Format: Patent
• Language: English; Spanish
• Published: 01.08.1981
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PHYSICS; SPIRITS; SUGARS; TESTING; VINEGAR; WINE

A chromatographic process for separation of an aminoglycoside antibiotic from impurities. Illustratively, there is disclosed a chromatographic process for separation of the gentamicin complex (C1, C2 and C1a) from impurities. The subject process gives a gentamicin complex recovery of more than double the recovery of the best known prior art gentamicin chromatography process. PROCEDIMIENTO CROMATOGRAFICO PARA LA SEPARACION DE IMPUREZAS DE UN ANIBIOTICO AMINOGLUCOSIDO. SE AJUSTA EL PH DE UNA SOLUCION DE ANTIBIOTICO AMINOGLUCOSIDO, QUE ES UN COMPLEJO DE GENTAMICINA, ALREDEDOR DE 3 A 11; SE PASA LA SOLUCION DE PH AJUSTADO POR UNA RESINA DE INTERCAMBIO IONICO QUE HA SIDO CALENTADA ENTRE 25 Y 100 GC; SE PASA AGUA A UNA TEMPERATURA DE 25 A 100 GC, POR UNA COLUMNA CROMATOGRAFICA; POR ULTIMO SE RECOGEN LAS FRACCIONES RICAS EN ANTIBIOTICO AMINOGLUCOSIDO, QUE A LA VEZ SON FRACCIONES RICAS EN GENTAMICINA.