Expansión mejorada de linfocitos infiltrantes de tumores para terapia celular adoptiva

• Main Authors: MULE, James J, SARNAIK, Amod A, WEBER, Jeffrey S, PILON-THOMAS, Shari, CHACON, Jessica Ann, RADVANYI, Laszlo G
• Format: Patent
• Language: Spanish
• Published: 03.10.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

En el presente documento se describe un método para expandir ex vivo linfocitos infiltrantes de tumores para su uso en terapia celular adoptiva (ACT). El método implica cultivar fragmentos de tumor del sujeto en un medio de cultivo que contiene IL-2 y un agonista de 41BB en una cantidad eficaz para expandir linfocitos infiltrantes de tumores con reactividad y especificidad tumorales enriquecidas. También se describe un método para tratar un tumor en un sujeto que implica tratar al sujeto con quimioterapia no mieloablativa de depleción linfoide y administrar linfocitos infiltrantes de tumores expandidos mediante los métodos descritos. (Traducción automática con Google Translate, sin valor legal) Disclosed herein is a method for ex vivo expanding tumor-infiltrating lymphocytes for use in adoptive cell therapy (ACT). The method involves culturing tumor fragments from the subject in a culture medium containing IL-2 and a 41BB agonist in an amount effective to expand tumor-infiltrating lymphocytes with enriched tumor-reactivity and specificity. Also disclosed is a method for treating a tumor in a subject that involves treating the subject with nonmyeloablative lymphodepleting chemotherapy, and administering tumor-infiltrating lymphocytes expanded by the disclosed methods.