Métodos y composiciones para la modificación genética dirigida a través de múltiples direccionamientos en un solo paso
Methods and compositions are provided for making one or more targeted genetic modifications at a target genomic locus within a cell and for producing non-human animals comprising the modified genomic locus. The methods employ two or more large targeting vectors which are capable of recombining with...
Saved in:
Main Authors | , , , |
---|---|
Format | Patent |
Language | Spanish |
Published |
14.03.2024
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Methods and compositions are provided for making one or more targeted genetic modifications at a target genomic locus within a cell and for producing non-human animals comprising the modified genomic locus. The methods employ two or more large targeting vectors which are capable of recombining with each other and with the target genomic locus in a single genomic targeting step. The methods may also be employed in combination with a nuclease agent. Methods and compositions are also provided for enhancing homologous recombination at a target genomic locus in a cell. The methods employ two or more nucleic acids comprising one or more overlapping sequences. The methods may be employed in combination with a nuclease agent or without a nuclease agent. |
---|---|
AbstractList | Methods and compositions are provided for making one or more targeted genetic modifications at a target genomic locus within a cell and for producing non-human animals comprising the modified genomic locus. The methods employ two or more large targeting vectors which are capable of recombining with each other and with the target genomic locus in a single genomic targeting step. The methods may also be employed in combination with a nuclease agent. Methods and compositions are also provided for enhancing homologous recombination at a target genomic locus in a cell. The methods employ two or more nucleic acids comprising one or more overlapping sequences. The methods may be employed in combination with a nuclease agent or without a nuclease agent. |
Author | MURPHY, Andrew VORONINA, Vera ZAMBROWICZ, Brian MACDONALD, Lynn |
Author_xml | – fullname: MACDONALD, Lynn – fullname: ZAMBROWICZ, Brian – fullname: MURPHY, Andrew – fullname: VORONINA, Vera |
BookMark | eNqNjTsOwjAQRFNAwe8OywEo-EhRahREQ0X6aGVvopVsrxUbJMSJUtPR-mIYiQNQjUYz82ZeTJw4mhXPSxqjaAnwACXWS2DFOQngcUAwCFY0d6xQcXo56MnlQbageeCeNQJCHPCexgCawKa3iexNBuQCqS8MLZOL-YIc3BwEMZLpQZbFtEMTaPXTRbE-1c3xvCEvLQWPihzFtr7uqkNZbg9NU-3_6XwAbapNTA |
ContentType | Patent |
DBID | EVB |
DatabaseName | esp@cenet |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: EVB name: esp@cenet url: http://worldwide.espacenet.com/singleLineSearch?locale=en_EP sourceTypes: Open Access Repository |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Chemistry Sciences Forestry Agriculture |
ExternalDocumentID | ES2947714TT9 |
GroupedDBID | EVB |
ID | FETCH-epo_espacenet_ES2947714TT93 |
IEDL.DBID | EVB |
IngestDate | Fri Jul 19 13:08:56 EDT 2024 |
IsOpenAccess | true |
IsPeerReviewed | false |
IsScholarly | false |
Language | Spanish |
LinkModel | DirectLink |
MergedId | FETCHMERGED-epo_espacenet_ES2947714TT93 |
Notes | Application Number: ES20190201384T |
OpenAccessLink | https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240314&DB=EPODOC&CC=ES&NR=2947714T9 |
ParticipantIDs | epo_espacenet_ES2947714TT9 |
PublicationCentury | 2000 |
PublicationDate | 20240314 |
PublicationDateYYYYMMDD | 2024-03-14 |
PublicationDate_xml | – month: 03 year: 2024 text: 20240314 day: 14 |
PublicationDecade | 2020 |
PublicationYear | 2024 |
RelatedCompanies | Regeneron Pharmaceuticals, Inc |
RelatedCompanies_xml | – name: Regeneron Pharmaceuticals, Inc |
Score | 3.534669 |
Snippet | Methods and compositions are provided for making one or more targeted genetic modifications at a target genomic locus within a cell and for producing non-human... |
SourceID | epo |
SourceType | Open Access Repository |
SubjectTerms | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TRAPPING VINEGAR WINE |
Title | Métodos y composiciones para la modificación genética dirigida a través de múltiples direccionamientos en un solo paso |
URI | https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240314&DB=EPODOC&locale=&CC=ES&NR=2947714T9 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV07T8MwED5BQTwmKCAoDxkJdasgqZvHECGapKqQ-hAE1K1yGqeKRJOqTkGIX9SZjTV_jHNIgQU2J5Yvju3L3TnffQa4UKjCQowDagGjGKBI9lZD04yapqt1rl-FDZ3nANmu1n6gt4PGYAWiZS5MzhP6kpMjokaNUN_T_Hs9_dnEcnJspbj0I7yVXLc8y6kW0bEkl1No1Wlabr_n9OyqbVvoVHXvLNWkuq5Qz1yFNelFS5p997Epk1Kmvy1KawfW-ygsTndhhYsybN-MZwULBi_Dpr08hq0MG_LwzKLYKX6EY7HQSbEHb51skWJwKcgrkQDxRO5USAZ-Imm9yRMjkySQgCA2irL3mOCKwQZ4SdCcReMoYISRdMaes4UgASeT7OMLYyhIbu-kMDaRaZP4CB6TeUxwvSYoXST7cN5yPbtdw9cbfg_l0L1fDoRn1g-gFGN3DoGozA-ZyZhJQ4OqYcNglHGMpUxf03X0eY6g8recyn-Vx7Alp0WithR6AqV0NuenaMZT_yyfgE9HhKfY |
link.rule.ids | 230,309,786,891,25594,76904 |
linkProvider | European Patent Office |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1NT4NAEJ3UaqyetGq0fq2J6a1R6BbKgRgLNFX7FUXjrVnK0pBYaArVGH9Rz9688secRape9LZAGJbdHWbf8uYtwKlEJeYhDqi4jCJAEeqtdUWpVxRVrnL13KupPCXIdpXWPb1-rD3mwF_kwqQ6oS-pOCJ61BD9PU6_15OfRSwz5VZGZ46Pp8KLpq2b5QwdC3E5iZbNhm71e2bPKBuGjpOq7q0ua1RVJWprS7CsIiIUMvvWQ0MkpUx-R5TmBqz00VgQb0KOR0VYvxxNMxUMXoSCsdiGrQirYvPMrNjJfoRjMfPJaAveOsk8RnAZkVciCOKhWKkQCvxEyHqTJ0bGoSsIQWzoJ-8BwRGDN-AhwXDmj3yXEUbiKXtO5hFxORknH18cw4ik8U4YY2ORNomP4AGZBQTHa4jWo3AbTpqWbbQq-HqD76YcWHeLhrC16g7kA6zOLhCZOR7TGNOoV6eyV6szyjhiKc1RVBXnPHtQ-ttO6b-Lx1Bo2Z32oH3VvdmHNdFFgsEl0QPIx9MZP8SQHjtHaWd8AkiMqsM |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Apatent&rft.title=M%C3%A9todos+y+composiciones+para+la+modificaci%C3%B3n+gen%C3%A9tica+dirigida+a+trav%C3%A9s+de+m%C3%BAltiples+direccionamientos+en+un+solo+paso&rft.inventor=MACDONALD%2C+Lynn&rft.inventor=ZAMBROWICZ%2C+Brian&rft.inventor=MURPHY%2C+Andrew&rft.inventor=VORONINA%2C+Vera&rft.date=2024-03-14&rft.externalDBID=T9&rft.externalDocID=ES2947714TT9 |