Métodos y composiciones para la modificación genética dirigida a través de múltiples direccionamientos en un solo paso

Methods and compositions are provided for making one or more targeted genetic modifications at a target genomic locus within a cell and for producing non-human animals comprising the modified genomic locus. The methods employ two or more large targeting vectors which are capable of recombining with...

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Bibliographic Details
Main Authors MACDONALD, Lynn, ZAMBROWICZ, Brian, MURPHY, Andrew, VORONINA, Vera
Format Patent
LanguageSpanish
Published 14.03.2024
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CARE OF BIRDS, FISHES, INSECTS
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW BREEDS OF ANIMALS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
SPIRITS
TRAPPING
VINEGAR
WINE
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Summary:Methods and compositions are provided for making one or more targeted genetic modifications at a target genomic locus within a cell and for producing non-human animals comprising the modified genomic locus. The methods employ two or more large targeting vectors which are capable of recombining with each other and with the target genomic locus in a single genomic targeting step. The methods may also be employed in combination with a nuclease agent. Methods and compositions are also provided for enhancing homologous recombination at a target genomic locus in a cell. The methods employ two or more nucleic acids comprising one or more overlapping sequences. The methods may be employed in combination with a nuclease agent or without a nuclease agent.
Bibliography:Application Number: ES20190201384T