Separación, disociación y/o desagregación de las células usando impactos mecánicos

• Main Authors: CHI, Ben-Chen, CHI, John, RAVEN, Raymond, RICHMAN, Mark
• Format: Patent
• Language: Spanish
• Published: 10.12.2021
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Un método para aislar células madre de tejido adiposo, que comprende: proporcionar una muestra de tejido adiposo de un sujeto; colocar la muestra de tejido en un recipiente o cartucho de procesamiento (205); someter la muestra de tejido a la fuerza de impactos mecánicos para liberar las células madre; y separar una fracción de células madre del tejido adiposo; en donde: la fuente de la fuerza de los impactos mecánicos es un brazo de impacto (310) que es accionado por un motor (305), comprendiendo el motor un engranaje capaz de reducir la velocidad del brazo de impacto (310) cuando el motor (305) está funcionando a toda velocidad; y el brazo de impacto (310) no contacta físicamente con el tejido adiposo. Methods provided by the present disclosure utilize extracorporeal shockwaves, mechanical impacts and/or principles of lithotripsy to break up a tissue sample into smaller fragments - clusters of cells and/or single cells - after which a desired cellular fraction can be isolated from the sample. Devices provided by the present disclosure deploy focused and/or directed shockwaves, and/or focused and directed mechanical impacts, to break apart a tissue sample. The devices maintain the sample in a sterile, closed environment during exposure to the shockwaves or mechanical impacts. Therefore, the shockwaves and/or mechanical impacts are generated outside of a closed device and are transmitted through one or more walls of the device into its interior, where the sample is located.