Mutantes recombinantes selectivos de Mycobacterium smegmatis mc2 155 y su uso para la producción de 1,4-androstadien-3,17-diona o 4-androsten-3,17-diona a partir de esteroles naturales

• Main Authors: GALAN SICILIA, Beatriz, UHIA CASTRO, Iria, GARCIA LOPEZ, José Luis
• Format: Patent
• Language: Spanish
• Published: 25.11.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

La presente invención se refiere a cepas bacterianas de la especie M. smegmatiscon la actividad 3-cetosteroide-9α-hidroxilasa inactivada o con las actividades 3- cetosteroide-9α-hidroxilasa y 3-cetosteroide-Δ1-deshidrogenasa, y usos de dichas cepas para la obtención de AD o ADD sustancialmente puros así como de esteroides a partir de los mismos. The present invention relates to bacterial strains of the species M. smegmatis with inactivated 3-ketosteroid-9±-hydroxylase activity or with 3-ketosteroid-9±-hydroxylase and 3-ketosteroid-”1-dehydrogenase activities, and uses of said strains for obtaining substantially pure AD or ADD, and for obtaining steroids from same.