Hidrogeles de tipo esponjoso de goma de gelano, preparación y aplicaciones biomédicas de los mismos

• Main Authors: PEREIRA DA SILVA, Lucília, TEIXEIRA CERQUEIRA, Mariana, GONSALVES DOS REIS, Rui Luís, PINTO MARQUES, Alexandra Margarida, CORRELO DA SILVA, Vítor Manuel, ROMERO AMANDI DE SOUSA, Rui Pedro
• Format: Patent
• Language: Spanish
• Published: 29.12.2017
• Subjects: AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OF MACROMOLECULAR COMPOUNDS; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; GENERAL PROCESSES OF COMPOUNDING; HUMAN NECESSITIES; HYGIENE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; WORKING-UP

Un material de goma de gelano adhesivo celular, en el que dicho material es un hidrogel de goma de gelano de tipo esponjoso con un diámetro de poro entre 10 μm y 900 μm, y un diámetro medio de poro entre 200 μm y 600 μm, un grosor de pared de poro que varía entre 50 μm y 100 μm y un contenido de agua entre el 1000 y el 2500 % (p/p). The present application discloses cell-adhesive gellan gum spongy-like hydrogels that are able to entrap/encapsulate adherent cells, which spread within the material, maintaining their phenotype and remaining viable and proliferative. The methodology used to obtain these materials involves hydrogel preparation, freezing, freeze-drying and re-hydration with a saline solution with cells and with/without bioactive molecules. No pre and/or post functionalization of the spongy-like hydrogels with cell adhesive features, as used for other hydrogels, is used. The cell adhesive character of these materials, not observed in hydrogels, is in part explained by their physical properties, between sponges and hydrogels, dissimilar from the precursor hydrogels. The physical properties that are mainly different are the morphology, microstructure, water content, and mechanical performance. Gellan gum spongy-like hydrogels physical properties and biological performance can be tuned by manipulating the parameters involved in spongy-like hydrogel formation. Bioactive molecules can also be entrapped with or without cells to modify the biological performance of the spongy-like hydrogels. These materials can be applied in the context of bioengineering, tissue engineering, regenerative medicine and biomedical applications.