Host cell myceliophthora thermophila with improved cellulolytic activity and enzymatic compositions obtained by it (Machine-translation by Google Translate, not legally binding)

• Main Authors: MARTIN PEREZ, LUCIA, GAVALDA MARTIN, SANDRA, DIEZ GARCIA, BRUNO, SANCHEZ ZAMORANO, LAURA, ARJONA ANTOLIN, RICARDO, REYES SOSA, FRANCISCO MANUEL, ALVAREZ NUNEZ, CONSOLACION, RAMOS MARTIN, JUAN LUIS, PEREZ GOMEZ, DOLORES, MORENO PEREZ, ANTONIO JAVIER, PLATERO GOMEZ, ANA ISABEL, VINAS DE LA CRUZ, LAURA, BERMUDEZ ALCANTARA, MARIA DE LOS ANGELES, LEDESMA GARCIA, LAURA, VALBUENA CRESPO, NOELIA, ROCHA MARTIN, JAVIER
• Format: Patent
• Language: English; Spanish
• Published: 20.12.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Host cell myceliophthora thermophila with improved cellulolytic activity and enzymatic compositions obtained therefrom. The present invention relates to a host cell, preferably a cell of myceliophthora thermophila, which shows a lower expression and/or secretion of non-contributive cellulolytic enzymes, preferably where the non-contributive cellulolytic enzyme is the endoglucanase 6 comprising seq id no: 2, thus favoring the presence of contributive cellulolytic enzymes in the enzymatic cocktail synthesized by said host cell. The present invention also relates to the use of said host cells and enzymatic cocktails synthesized by said host cells for obtaining fermentable sugars from the biomass and a process for producing bioproducts, preferably bioethanol, comprising the use of said host cell or of the composition of the invention. (Machine-translation by Google Translate, not legally binding) Célula hospedadora Myceliophthora thermophila con actividad celulolítica mejorada y composiciones enzimáticas obtenidas por la misma. La presente invención se refiere a una célula hospedadora, preferiblemente una célula de Myceliophthora thermophila, que muestra una menor expresión y/o secreción de enzimas celulolíticas no contributivas, preferiblemente donde la enzima celulolítica no contributiva es la endoglucanasa 6 que comprende la SEQ ID NO: 2, favoreciendo así la presencia de enzimas celulolíticas contributivas en el cóctel enzimático sintetizado por dicha célula hospedadora. La presente invención también se refiere al uso de dichas células hospedadoras y de los cócteles enzimáticos sintetizados por dichas células hospedadoras para la obtención de azúcares fermentables de la biomasa y un procedimiento para producir bioproductos, preferiblemente bioetanol, que comprende el uso de dicha célula hospedadora o de la composición de la invención.