Procedure for the detection of adulterations of saffron with gardenia based on the determination of geniposides (Machine-translation by Google Translate, not legally binding)

• Main Authors: GUIJARRO DIEZ, Miguel, CASTRO PUYANA, María, MARINA ALEGRE, María Luisa, CREGO NAVAZO, Antonio Luis
• Format: Patent
• Language: English; Spanish
• Published: 05.09.2017
• Subjects: FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; TESTING; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES

The objective of the invention is the development of a procedure for the detection of adulterations of saffron with gardenia based on the determination of geniposide by high performance liquid chromatography with detection by mass spectrometry. The presence of geniposide in extracts of gardenia and its absence in saffron allows proposing said compound as a marker of adulteration of saffron with gardenia. The invention consists in the chromatographic separation of geniposides from the rest of the components of saffron samples using a c18 column and an isocratic elution with a mobile phase of water: acetonitrile (85:15 v/v) with 0.1% formic acid and detection by mass quadrupole-time-of-flight spectrometry. (Machine-translation by Google Translate, not legally binding) El objetivo de la invención es el desarrollo de un procedimiento para la detección de adulteraciones de azafrán con gardenia basado en la determinación de genipósido por cromatografía líquida de alta eficacia con detección por espectrometría de masas. La presencia de genipósido en extractos de gardenia y su ausencia en el azafrán permite proponer dicho compuesto como marcador de adulteración de azafrán con gardenia. La invención consiste en la separación cromatográfica de genipósido del resto de componentes de las muestras de azafrán utilizando una columna C18 y una elución isocrática con una fase móvil de agua: acetonitrilo (85:15 v/v) con 0.1% de ácido fórmico y detección por espectrometría de masas de quadrupolo-tiempo de vuelo.