Método analítico para moléculas Fab y Fab

• Main Authors: KIRKE, Helen Marie, SMITH, Bryan John
• Format: Patent
• Language: Spanish
• Published: 23.06.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

Un método de medición de especies ácidas generadas por la degradación de un componente de Fab' de un Fab'- PEG que comprende las etapas de: a) dividir el PEG y el enlazador del Fab'-PEG con una enzima que divide la porción de bisagra del Fab' y libera el PEG y el enlazador del Fab', b) separar el PEG y el enlazador generado en la etapa a) del Fab', para proporcionar un Fab' y c) analizar cuantitativamente las especies ácidas asociadas con el Fab' dividido. A method of measuring acidic species generated by degradation of a Fab or Fab′ component of a Fab-PEG or a Fab′-PEG is provided. The method involves: a) cleaving PEG and a linker from the Fab-PEG or Fab′-PEG with an enzyme; b) optionally separating the PEG and linker from the Fab or Fab′ to provide isolated Fab or Fab′; and c) quantitatively analyzing acidic species associated with the cleaved Fab or Fab′ and/or the cleaved PEG.