Producción de semillas híbridas

• Main Authors: DALY, ALLAN, BAYLISS, MICHAEL WILLIAM, JEPSON, IAN, KNIGHT, MARY ELIZABETH
• Format: Patent
• Language: Spanish
• Published: 20.07.2012
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

Un casete de expresión que comprende:(a) un primer promotor génico que es un promotor específico de tapetum, de antera, de estambre y/o de polen;(b) un gen interruptor que codifica un producto capaz de interrumpir la fertilidad masculina, operablementeenlazado al primer promotor génico;(c) un segundo promotor génico que es un promotor específico floral de ovario, de óvulos, de pistilo, de estilo, deestigma, de aparato transmisor y/o de placenta, opcionalmente enlazado operablemente a una o mássecuencias potenciadoras de la traducción o intrónicas;(d) un gen restaurador que codifica un producto capaz de restaurar la fertilidad femenina, operablementeenlazado al segundo promotor génico;(e) un tercer promotor génico que es inducible mediante la aplicación de un inductor químico exógeno,opcionalmente enlazado operablemente a una o más secuencias potenciadoras de la traducción o intrónicas; y(f) un gen restaurador que codifica un producto capaz de restaurar la fertilidad masculina, operablementeenlazado al tercer promotor génico. Methods of preparing hybrid seed are described. One such method comprises interplanting a male parent plant which is male fertile and homozygous recessive female sterile and a female parent plant which is homozygous recessive male sterile and female fertile, allowing cross-pollination and obtaining the seed produced therefrom. The genomic material of each parent plant may also have integrated therein a gene construct comprising a promoter sequence responsive to the presence or absence of an exogenous chemical inducer, optionally operably linked to one or more enhancer or intron sequences, operably linked to a gene which fully restores the fertility of each parent plant, the gene being expressed by the application to the plant of an external chemical inducer thereby allowing each parent to self-pollinate.