Method for producing mesenchimal truncal cells derived from adipose tissue, as well as mesenquimal trunk cells obtained by such method. (Machine-translation by Google Translate, not legally binding)

• Main Authors: RODRIGUEZ-ALTONAGA MARTINEZ, JOSE ANTONIO, RAMOS PASCUA, LUIS RAFAEL, GONZALO ORDEN, JOSE MANUEL, IGLESIAS MUNOZ, JAVIER, SALDANA DIAZ, ANDRES, LOPEZ LAGUNA, MARTA
• Format: Patent
• Language: English; Spanish
• Published: 17.01.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Method to produce mesenchymal stem cells derived from adipose tissue, as well as the mesenchymal stem cells obtained by said method. The present invention describes a method for producing mesenchymal stem cells derived from adipose tissue obtained from mammals comprising the steps of obtaining the sample of adipose tissue, purifying and fragmenting thereof; isolation of mesenchymal stem cells; in vitro culture and expansion; characterization and differentiation of the cell population by adipogenic, osteogenic or chondrogenic induction to promote their differentiation into cells of adipose, bone or cartilaginous tissue. The sample of adipose tissue is maintained at room temperature before being subjected to enzymatic digestion, keeping the mixture at rest and avoiding filtering. The cells obtained, and in particular those of osteogenic phenotype can be used for bone regeneration. (Machine-translation by Google Translate, not legally binding) Método para producir células troncales mesenquimales derivadas de tejido adiposo, así como las células troncales mesenquimales obtenidas por dicho método.La presente invención describe un método para producir células troncales mesenquimales derivadas de tejido adiposo obtenido de mamíferos que comprende las fases de obtención de la muestra de tejido adiposo, purificación y fragmentación de la misma; aislamiento de las células troncales mesenquimales; cultivo y expansión in vitro; caracterización y diferenciación de la población celular por inducción adipogénica, osteogénica o condrogénica para promover su diferenciación hacia células de tejido adiposo, óseo o cartilaginoso. La muestra de tejido adiposo se mantiene a temperatura ambiente antes de ser sometida a digestión enzimática, manteniendo la mezcla en reposo y evitando el filtrado. Las células obtenidas, y en particular, las de fenotipo osteogénico pueden utilizarse para regeneración ósea.