HEAT-RESISTANT ENDOXYLANASE OBTAINED BY MUTAGENESIS AND THE USE THEREOF IN THE BIOETHANOL PRODUCTION PROCESS

• Main Authors: MARTORELL GUEROLA, PATRICIA, SANCHEZ HERVAS, MERITXELL, MANZANARES MIR, PALOMA, VALLES ALVENTOSA, SALVADOR, MC CABE, ANDREW PETER, GENOVES MARTINEZ, SALVADOR, POLAINA MOLINA, JULIO, RAMON VIDAL, DANIEL
• Format: Patent
• Language: English; Spanish
• Published: 29.12.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention describes a peptide sequence in which a series of amino acids has been substituted by site-directed mutagenesis from the sequence encoding Aspergillus nidulans endoxylanase, in order to obtain an endoxylanase that has greater thermal stability. Furthermore, the present invention relates to the use of this endoxylanase for obtaining the xylan from plant material and to the subsequent use of this xylan in bioethanol production processes. Endoxilanasa termorresistente obtenida por mutagénesis y su aplicación al proceso de obtención de bioetanal.La presente invención describe una secuencia peptídica a la se han sustituido una serie de aminoácidos por mutagénesis dirigida a partir de la secuencia codificante de endoxilanasa de Aspergíllus nidulans para obtener una endoxilanasa que presenta una mayor estabilidad térmica. Además, la presente invención se refiere al uso de esta endoxilanasa para la obtención de xilano a partir de materia vegetal y al posterior uso de este xilano en procesos de obtención de bioetanol.