POLYNUCLEOTIDE COMPRISING SEQUENCES OF WHEAT GLIADINS AND USE THEREOF FOR SILENCING BY RNAI

• Main Authors: GIL HUMANES JAVIER, PISTON PISTON FERNANDO, MARTIN MUNOZ ANTONIO, BARRO LOSADA FRANCISCO
• Format: Patent
• Language: English; Spanish
• Published: 04.08.2010
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TRAPPING; VINEGAR; WINE

The present invention relates to the specific silencing of the +- (alpha, (beta), (gamma) and É (omega)-gliadins of hard wheat for flour by RNA interference (RNAi) through employment of a polynucleotide which is transcribed into an hpRNA (hairpin RNA). Furthermore the present invention additionally relates to a vector, cell, plant or seed comprising the polynucleotide, the expression whereof is specifically directed in particular tissues of wheat seeds through gene expression-regulating sequences such as, for example, the promoter of a gene of -gliadins or the promoter of the gene encoding for a D-hordein. Polinucleótido que comprende secuencias de gliadinas de trigo y su uso para silenciamiento mediante RNAi. La presente invención se refiere al silenciamiento especifico de las · (alfa), · (beta), · (gamma) y o·(omega)-gliadinas de trigo duro y harinero mediante RNA de interferencia (ARNi) por medio del empleo de un polinucleótido que se transcribe a un hpRNA (hairpin RNA). Además, la presente invención también se refiere a un vector, célula, planta o semilla que comprenden el polinucleótido, cuya expresión se dirige de forma específica en tejidos concretos de las semillas de trigo mediante secuencias reguladoras de la expresión génica como por ejemplo, el promotor de un gen de ·-gliadinas o el promotor del gen que codifica para una D-hordeína.