CONJUNTO DE CEBADORES, SONDAS, PROCEDIMIENTO Y KIT PARA EL GENOTIPADODEL POLIMORFISMO GENETICO S447X DEL GEN LPL

• Main Authors: VALDIVIELSO FELICES, PEDRO, SANCHEZ CHAPARRO, MIGUEL ANGEL, ARIZA CORBO, MARIA JOSE, GONZALEZ SANTOS, PEDRO, RIOJA VILLODRES, JOSE
• Format: Patent
• Language: Spanish
• Published: 19.07.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Set of primers, probes, procedure and kit for the genotyping of the s447x genetic polymorphism of the lpl gene. The present invention relates to a set of primers, probe, method and kit for genotyping genetic polymorphisms, and more specifically for the genotyping of the s447x genetic polymorphism of the lpl gene by pcr amplification of a dna sample using the set of primers claimed, and the detection by fluorescence and genotyping of the genetic polymorphism using the set of probes claimed. The main advantages of the present invention are: great rapidity that allows genotyping on a large scale since the pcr reaction and the detection of the fluorescent signal are simultaneous, automated genotyping and obtainable immediately after the end of the reaction, great sensitivity that allows genotyping samples using very low dna concentrations, and lower risk of contamination since it is a homogeneous assay. (Machine-translation by Google Translate, not legally binding)