SPECIFIC PROMOTER OF FRUIT OF SOLANUM LYCOPERSICUM

• Main Authors: PINEDA CHAZA BENITO, GRANELL RICHART ANTONIO, ORZAEZ CALATAYUD DIEGO, HUESO ESTORNELL LEANDRO, ANTON MARTINEZ TERESA, MORENO FERRERO VICENTE
• Format: Patent
• Language: English; Spanish
• Published: 10.02.2010
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The present invention relates to a promoter sequence denominated FSP1 (SEQ. ID. NO.:2) situated in region 5' of the DNA of gene Sn1 of the tomato Solanum lycopersicum. The invention also relates to constructions of nucleic acid, vectors, plant cells and transgenic plants comprising the promoter together with seeds and fruit thereof. The promoter may be used to direct sense, antisense, miRNA or RNAi gene expression specifically in tissues of the fruit. Although other specific fruit promoters in tomato have been described, tomato FSP1 is particularly useful when it is desired to use a tomato promoter conferring specific expression on the fruit at different stages, but particularly during ripening and specifically in the tissues of the columella, placenta and the interlocular gel, and when it is desired that the resulting levels of expression are moderate or low. La presente invención se refiere a una secuencia de promotor denominada FSP1 (SEQ ID NO:2), situada en la región 5'' del DNA del gen Sn1 de tomate Solanum lycopersicum. La invención también se refiere a construcciones de ácido nucleico, vectores, células de plantas y plantas transgénicas que comprenden el promotor, así como a las semillas y los frutos de las mismas. El promotor puede usarse para dirigir la expresión génica sentido, antisentido, miRNA o RNAi específicamente en tejidos del fruto. Aunque se han descrito otros promotores específicos de fruto en el tomate, el FSP1 de tomate es particularmente útil cuando se desea usar un promotor de tomate que confiera expresión específica en el fruto en diferentes estadios pero especialmente durante la maduración y específicamente en los tejidos de la columnela, placenta y gel interlocular y cuando se desea que los niveles de expresión resultantes sean moderados o bajos.