CONSTITUTIVE PROMOTER OF SOLANUM LYCOPERSICUM

• Main Authors: PINEDA CHAZA BENITO, GRANELL RICHART ANTONIO, ORZAEZ CALATAYUD DIEGO, HUESO ESTORNELL LEANDRO, ANTON MARTINEZ TERESA, MORENO FERRERO VICENTE
• Format: Patent
• Language: English; Spanish
• Published: 10.02.2010
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to a promoter sequence (SEQ. ID. N0:2) derived from a gene of Solanum lycopersicum, of an unknown function and denominated CNH1. The invention relates to nucleotidic constructions, vectors, cells of plants and transgenic plants comprising the promoter of the invention, and the seeds thereof. The constitutive promoter according to the invention can be used for the sense, miRNA, antisense or RNAi gene expressions of the DNA sequences with which it merges, in all of the organs of the plant, with especially high levels in fruit. The promoter is especially interesting when expression levels similar to those obtained by the promoter CaMV 35S are desired in the fruit over different stages of its development, but not at such a high level in the rest of the plant, especially in Solanaceae/Solanum, enabling the use of heterologous promoters to be avoided. La presente invención se refiere a una secuencia promotora (SEQ ID NO:2), derivada de un gen de Solanum lycopersicum, de función desconocida y denominado CNH1. La invención está dirigida a las construcciones nucleotídicas, vectores, células de plantas y plantas transgénicas que comprenden el promotor de la invención, así como a sus semillas. El promotor constitutivo de la invención es útil para la expresión génica sentido, miRNA, antisentido o RNAi de las secuencias de DNA a las que se fusiona, en todos los órganos de la planta, con niveles especialmente elevados en el fruto. El promotor es de especial interés cuando niveles de expresión similares a los obtenidos por el promotor CaMV 35S son deseados en el fruto durante diferentes estadios de su desarrollo, pero no de forma tan elevada en el resto de la planta, especialmente dentro de Solanaceae/Solanum, permitiendo evitar el empleo de promotores heterólogos.