ATTENUATED LIVE STRAIN OF SALMONELLA ENTERICA SEROVAR CHOLERAESUIS AS A VACCINE AND VACCINAL VEHICLE FOR SWINE LIVESTOCK

• Main Authors: MARTINEZ PULGARIN SUSANA, DE LA FUENTE LOPEZ RICARDO, TIERREZ MARTINEZ ALBERTO, ORDEN GUTIERREZ JOSE ANTONIO, BARTOLOME AVARZA ALMIRA, DOMINGUEZ BERNAL GUSTAVO
• Format: Patent
• Language: English; Spanish
• Published: 24.07.2009
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Attenuated live strain of Salmonella Enterica Serovar Choleraesuis as a vaccine and vaccinal vehicle for swine livestock. The present invention provides a novel live and attenuated vaccinal strain, incapable of producing the alternative sigma factor (RpoS) and which, functions as a vaccinal vehicle for heterologous antigens. The strain is constructed by means of phase deletion of the totality of the gene which codifies the alternative sigma factor, rpoS, by means of homologous recombination. The strain is avirulent, genetically stable, lacks antibiotic resistance markers and is easily differentiated from wild strains. Cepa viva atenuada de Salmonella enterica serovar Choleraesuis como vacuna y vehículo vacunal para el ganado porcino. La presente invención proporciona una nueva cepa vacunal viva y atenuada, incapaz de producir el factor alternativo sigma (RpoS) y que, además, funciona como vehículo vacunal de antigenos heterólogos. La construcción de la cepa se ha realizado mediante la deleción en fase de la totalidad del gen que codifica el factor alternativo sigma, rpoS, mediante doble recombinación homóloga. La cepa es avirulenta, genéticamente estable, sin marcadores de resistencia antibiótica y se diferencia fácilmente de las cepas silvestres.