Method of quantification of damage or oxidative/nitrative/nitroactive stress (Machine-translation by Google Translate, not legally binding)

• Main Authors: LARRAINZAR RODRIGUEZ ESTIBALIZ, ARRESE-IGOR SANCHEZ CESAR, RODRIGUEZ URARTE ESTIBALIZ, GONZALEZ GARCIA ESTHER MARIA, MORAN JUEZ JOSE FERNANDO, AUZMENDI MIGUELENA INIGO
• Format: Patent
• Language: English; Spanish
• Published: 01.04.2009
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Method of quantification of damage or oxidative/nitrative/nitrosative stress. The present invention describes a method of in vitro quantification of oxidative and/or nitrative and/or nitrosative damage or stress, comprising the evaluation of the capacity of a cell, group of cells, tissue or organ of any living organism to stimulate production of reactive species of oxygen and/or nitrogen, and/or the estimation of the concentration of said reactive species, by means of the evaluation of the activity and/or level of a recombinant sod enzyme, as a consequence of the influence exerted by said reactive species of oxygen and/or nitrogen on the activity and level of said enzyme. (Machine-translation by Google Translate, not legally binding) Método de cuantificación del daño o estrés oxidativo/nitrativo/nitrosativo. La presente invención describe un método de cuantificación in vitro del daño o estrés oxidativo y/o nitrativo y/o nitrosativo, que comprende la evaluación de la capacidad de una célula, grupo de células, tejido u órgano de cualquier organismo vivo para estimular la producción de especies reactivas de Oxígeno y/o Nitrógeno, y/o la estimación de la concentración de dichas especies reactivas, mediante la valoración de la actividad y/o nivel de una enzima SOD recombinante, como consecuencia de la influencia ejercida por dichas especies reactivas de Oxígeno y/o Nitrógeno sobre la actividad y nivel de dicha enzima.