VECTOR DE ADENOVIRUS RECOMBINANTE Y METODOS DE USO

• Main Authors: MANEVAL, DANIEL C, GREGORY, RICHARD J, WILLS, KEN N
• Format: Patent
• Language: Spanish
• Published: 01.02.2007
• Edition: 4
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; SUGARS; VINEGAR; WINE

ESTA INVENCION PROPONE UN VECTOR RECOMBINANTE DE EXPRESION DE ADENOVIRUS CARACTERIZADO POR LA DELECION PARCIAL O TOTAL DE LA PROTEINA ADENOVIRAL IX ADN, QUE PRESENTA UN GEN QUE CODIFICA UNA PROTEINA EXTRAÑA O UN FRAGMENTO FUNCIONAL O UN MUTANTE DEL MISMO. LA INVENCION TAMBIEN INCLUYEN CELULAS HUESPED TRANSFORMADAS Y UN METODO PARA PRODUCIR PROTEINAS RECOMBINANTES Y TERAPIA GENICA. ASI POR EJEMPLO, EL VECTOR ADENOVIRAL DE ESTA INVENCION PUEDE CONTENER UN GEN EXTRAÑO PARA EXPRESAR UNA PROTEINA EFICAZ PARA REGULAR EL CICLO CELULAR, COMO P53, RB O MITOSINA, O PARA INDUCIR LA MUERTE CELULAR, COMO EL GEN SUICIDA CONDICIONAL DE TIMIDINA QUINASA (ESTE ULTIMO DEBE UTILIZARSE JUNTO CON UN METABOLITO DE TIMIDINA QUINASA PARA QUE RESULTE EFICAZ). This invention provides a recombinant adenovirus expression vector comprising a total deletion of a protein IX DNA and a gene encoding an anti-tumor protein. A pharmaceutical composition comprising said vector and its use for the treatment of cancer, especially hepatocellular carcinoma, are also part of the invention.