MEDIO DE CULTIVO PARA LA DETECCION DE CLOSTRIDIUM PERFRINGENS

• Main Authors: GARRIDO VAZQUEZ MANUEL JOAQUIN, GOMEZ LOPEZ MARIANO JULIO, ARAUJO PRADO MANUEL
• Format: Patent
• Language: Spanish
• Published: 01.03.2007
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Medio de cultivo para la detección de Clostridium perfringens. Comprende la disolución de proteosa peptona, peptona microbiológica, extracto de levadura, disulfito sódico y citrato férrico amónico en agua desionizada. Tras la esterilización a 121ºC /15 minutos y su enfriamiento, se completa con la adición de cicloserina y la sal disódica del 4-metilumbeliferil fosfato disueltas en agua desionizada y esterilizadas por filtración. El medio de cultivo se reparte en frascos de 150 mL de capacidad nominal, a razón de 50 mL, para su conservación en frigorífico y utilización antes de 15 días desde la fecha de preparación. Es de aplicación en la detección de Clostridium perfringens en aguas tras la inoculación de 100 mL de muestra en los frascos con 50 mL de medio y la incubación a 45ºC durante 18-20 h en condiciones aerobias. The invention relates to a culture medium for detecting Clostridium perfringens. It comprises dissolving proteose peptone, microbiological peptone, yeast extract, sodium disulfite and ammonium ferric citrate in deionized water. After sterilization at 121°C/15 minutes and cooling, it is completed with the addition of cycloserine and 4-methylumbelliferyl phosphate disodium salt dissolved in deionized water and sterilized by filtration. The culture medium is distributed into 150 mL bottles with a nominal capacity of 50 mL for its storage in refrigeration and its use no more than 15 days after its preparation date. It is applicable in detecting Clostridium perfringens in water after the inoculation of 100 mL of sample in the bottles with 50 mL of medium and the incubation at 45°C for 18-20 hours in aerobic conditions.