Procedure is for cultivation of recombinant Pseudomonas for use in desulfurizing dibenzothiophene

• Main Authors: ALCON MARTIN ALMUDENA, GARCIA-OCHOA SORIA FELIX, MARTIN EZQUERRA ANA BELEN, GARCIA CALVO ELOY, GARCIA LOPEZ JOSE LUIS, SANTOS MAZORRA VICTORIA E
• Format: Patent
• Language: English; Spanish
• Published: 16.05.2005
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CRACKING HYDROCARBON OILS; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FUELS; LUBRICANTS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MINERAL WAXES; MUTATION OR GENETIC ENGINEERING; PEAT; PETROLEUM, GAS OR COKE INDUSTRIES; PRODUCTION OF LIQUID HYDROCARBON MIXTURES, e.g. BY DESTRUCTIVEHYDROGENATION, OLIGOMERISATION, POLYMERISATION; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECOVERY OF HYDROCARBON OILS FROM OIL-SHALE, OIL-SAND, ORGASES; REFINING MIXTURES MAINLY CONSISTING OF HYDROCARBONS; REFORMING OF NAPHTHA; SPIRITS; TECHNICAL GASES CONTAINING CARBON MONOXIDE; VINEGAR; WINE

The procedure is for cultivation of recombinant Pseudomonas for use in desulfurizing dibenzothiophene. In a fermenter, the recombinant strain of Pseudomonas is produced, which is used as bio-catalyst for desulfurizing dibenzothiophene. A pre-inoculum is prepared in Luria-Bertani medium and an aerated commercial reactor using basal saline medium with 20 g/L of glutamic acid at a temperature of 30 degrees Celsius. The expression of the genes of the desulfurization route 4S is induced at the end of 4 hours from the commencement of the production of the micro-organism. The procedure is for cultivation of recombinant Pseudomonas for use in desulfurizing dibenzothiophene. In a fermenter, the recombinant strain of Pseudomonas is produced, which is used as bio-catalyst for desulfurizing dibenzothiophene. A pre-inoculum is prepared in Luria-Bertani medium and an aerated commercial reactor using basal saline medium with 20 g/L of glutamic acid at a temperature of 30 degrees Celsius. The expression of the genes of the desulfurization route 4S is induced at the end of 4 hours from the commencement of the production of the micro-organism. (Machine-translation by Google Translate, not legally binding) Procedimiento de cultivo de la Pseudomonas putida recombinante (CECT 5279) para su utilización en la desulfuración de dibenzotiofeno. La invención consiste en un procedimiento para producir en un fermentador la cepa recombinante de Pseudomonas putida CECT5279 que se utiliza como biocatalizador para desulfurar dibenzotiofeno. Se realiza la preparación de un preinóculo en medio LB (Luria - Bertani) y la producción en biorreactor comercial aireado empleando medio basal salino (BSM) con 20g/L de ácido glutámico a una temperatura de 30°C. La expresión de los genes de la ruta de desulfuración 4S se induce al cabo de 4 horas de haber comenzado la producción del microorganismo, empleando para ello IPTG. Esta invención indica, por tanto, el medio de cultivo y las condiciones de operación adecuadas para conseguir mejorar y aumentar la escala de producción de este biocatalizador con vistas a su utilización a nivel industrial.