MAIZE NUCLEOTIDE SEQUENCE CODING FOR A PROTEIN WITH TRANSGLUTAMINASE ACTIVITY AND USE THEREOF

• Main Authors: TALAVERA BARO DAVID, RIGAU LLOVERAS JUAN, VILLALOBOS AMADOR ENRIQUE, SANTOS LOZANO MARIA ASUNCION, TORNE CUBIRO JOSE MARIA
• Format: Patent
• Language: English; Spanish
• Published: 16.02.2005
• Edition: 7
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TRAPPING; VINEGAR; WINE

The invention relates to a DNA molecule from maize which codes for a protein with TGase activity and to a gene expression vector comprising said DNA molecule. The invention also relates to the use of the aforementioned DNA molecule or vector in order to produce transformed cells capable of expressing recombinant proteins with TGase activity and to introduce the sequence coding for a protein with TGase activity into plant cells. In addition, the invention relates to the resulting transgenic plants and cells of microorganisms. Furthermore, the proteins with TGase activity expressed from the above-mentioned DNA sequences can be used, for example, in food manipulation, processing and transformation. Secuencia de nucleotidos de maíz codificante de una proteína con actividad transglutaminasa, y su uso. La invención proporciona una molécula de ADN proveniente de maíz y codificante de una proteína con actividad TGasa, así como un vector de expresión génica que comprende esta molécula de ADN. Un objeto adicional de esta invención lo constituye el empleo de dicha molécula de ADN o vector para producir células transformadas capaces de expresar proteínas recombinantes con actividad TGasa, o para introducir dicha secuencia codificante de una proteína con actividad TGasa en células de plantas. Las células de microorganismos y las plantas transgénicas resultantes también constituyen objetos adicionales de esta invención. Además, estas proteínas con actividad TGasa expresadas a partir de dichas secuencias de ADN pueden ser empleadas, entre otros usos, en la manipulación, procesamiento y transformación de alimentos.