AGENTES ENTRECRUZANTS, CONJUGANTES Y REDUCTORES ACTIVADOS POR FOSFATASA; PROCEDIMIENTOS DE UTILIZACION DE ESTOS AGENTES Y REACTIVOS QUE COMPRENDEN ESTOS AGENTES

• Main Authors: HUSAIN, MAZHAR, YOUNG, DOUGLAS, F, SKRZYPCZYNSKI, ZBIGNIEW, BIENIARZ, CHRISTOPHER, CORNWELL, MICHAEL, J
• Format: Patent
• Language: Spanish
• Published: 01.01.2004
• Edition: 7
• Subjects: ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM; CHEMISTRY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; TESTING

LA PRESENTE INVENCION PROPORCIONA AGENTES DE RETICULACION, CONJUGACION Y REDUCCION QUE SON FUNCIONALES CON AL MENOS UN GRUPO MONOESTER FOSFOROTIOATO (- SPO{SUB,3}{SUP,-2}). TAMBIEN SE FACILITAN METODOS DE RETICULACION Y CONJUGACION ASI COMO REACTIVOS Y CONJUGADOS DE FASE SOLIDA QUE SON UTILES EN INMUNOENSAYOS. LOS AGENTES DE RETICULADO Y CONJUGACION DE LA INVENCION GENERALMENTE INCLUYEN UN COMPUESTO CORRESPONDIENTE A LA FORMULA (I): Q - (S - PO{SUB,3}{SUP,-2}){SUB,N}, DONDE N ES AL MENOS 1 Y Q ES UN MONOMERO, POLIMERO U OLIGOMERO LINEAL O RAMIFICADO, CON UN PESO MOLECULAR PROMEDIO ENTRE 200 Y 1.000.000. POR OTRA PARTE, CUANDO N ES 1, Q INCLUYE AL MENOS 1 GRUPO FUNCIONAL REACTIVO ADICIONAL. LOS AGENTES REDUCTORES PROPORCIONADOS SON COMPUESTOS DE FORMULA (Y) DONDE (A) Y (Z) PUEDEN SER UN ALQUILO C{SUB,1} - C{SUB,5} O CONH(CH{SUB,2}){SUB,P) DONDE P ES UN NUMERO ENTERO ENTRE 1 Y 5. The present invention provides crosslinking, conjugating and reducing agents which are functional with at least one phosphorothioate monoester group (-SPO3-2). Crosslinking and conjugation methods as well as solid phase reagents and conjugates which are useful in immunoassays are also provided. Crosslinking and conjugating agents of the invention generally comprise a compound corresponding to the formula (I), shown below, wherein n is at least 1 and Q is a straight or branched monomer, polymer or oligomer having an average molecular weight between about 200 and about 1,000,000. Additionally, when n is 1, Q comprises at least 1 additional reactive functionality.Q-(S-PO3-2)n(I)The reducing agents that are provided conform to a compound of the formula (Y), shown below, wherein (A) and (Z) can be independently selected from C1-C5 alkyl and CONH(CH2)p wherein p is an integer between 1 and 5.