FV MONOCATENARIOS ANTI-EGFR Y ANTICUERPOS ANTI-EGFR

• Main Authors: ADAN, JAIME, KETTLEBOROUGH, A. CATHRINE, MITJANS, FRANCESC, GUSSOW, DETLEF, BENDIG, MARY M, ANSELL, KEITH H
• Format: Patent
• Language: Spanish
• Published: 16.09.2003
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

LA INVENCION SE REFIERE A NUEVOS ANTICUERPOS ANTI-EGFR Y A CADENAS SIMPLES FVS (SCFVS) DE LOS MISMOS QUE PUEDEN OBTENERSE A PARTIR DE LIBRERIAS DE ANTICUERPOS CONSTRUIDAS A PARTIR DE CELULAS DE UN MAMIFERO INMUNIZADO, PREFERIBLEMENTE UN RATON. DOS DE LOS FVS DE CADENA SIMPLE AISLADOS A PARTIR DE LAS LIBRERIAS DE ANTICUERPOS FUERON MODIFICADOS GENETICAMENTE PARA CREAR MOLECULAS COMPLETAS DE ANTICUERPOS PARCIALMENTE HUMANIZADOS. ESTOS ANTICUERPOS QUIMERICOS ANTI-EGFR CONTIENE REGIONES CONSTANTES DE INMUNOGLOBULINAS HUMANAS Y PUEDEN UTILIZARSE ASI COMO LOS FVS DE CADENA SIMPLE COMO AGENTES PARA LA DIAGNOSIS Y LA TERAPIA DE TUMORES HUMANOS. PCT No. PCT/EP95/00978 Sec. 371 Date Nov. 17, 1995 Sec. 102(e) Date Nov. 17, 1995 PCT Filed Mar. 16, 1995 PCT Pub. No. WO95/25167 PCT Pub. Date Sep. 21, 1995This invention relates to new anti-EGFR antibodies and single-chain Fvs (scFvs) thereof which can be obtained from phage-antibody libraries constructed from cells of an immunized mammalian, preferably a mouse. Two of the single-chain Fvs isolated from the phage-antibody libraries were engineered to create partially humanized whole antibody molecules. These chimeric anti-EGFR antibodies contain constant regions of human immunoglobulins, and can be used as well as the single-chain Fvs as agents for the diagnosis and therapy of human tumors.