Protease activity stimulation by accelerated hydrolysis consists of trypsin based hydrolysis treatment of phospholipase

• Main Authors: LOPEZ BUESA PASCUAL, VERCET TORMO ANTONIO, ORIA ALMUDI ROSA
• Format: Patent
• Language: English; Spanish
• Published: 01.08.2003
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Protease activity stimulation by accelerated hydrolysis comprises trypsin-based hydrolysis of substrates at temperatures where they resist trypsin treatment. The treatment accelerates phospholipase hydrolysis at 60 [deg] C, but manothermosonication accompanying the treatment also has these effects at 75 and 90 [deg] C. Stimulation of proteases activity using manothermosonication.Proteases have many applications in the food, detergent, leather and other industries. Stimulating their activity under conditions in which this does not occur could help to increase their industrial use. Subjected to manothermosonication, trypsin hydrolyses substrates at temperatures at which said substrates are resistant to trypsin treatment. Manothermosonication accelerates phospholipase hydrolysis at 60 degrees C. At 75 degrees C and 90 degrees C, phospholipase cannot be hydrolysed by trypsin, but if the medium is subjected to manothermosonication, trypsin does hydrolyse it. Phospholipase always resists manothermosonication in the absence of trypsin.Protease activity stimulation by accelerated hydrolysis comprises trypsin-based hydrolysis of substrates at temperatures where they resist trypsin treatment. The treatment accelerates phospholipase hydrolysis at 60 [deg] C, but manothermosonication accompanying the treatment also has these effects at 75 and 90 [deg] C. (Machine-translation by Google Translate, not legally binding) Las proteasas tienen muchas aplicaciones en la industria alimentaria, de detergentes, del cuero etc. Estimular su actividad en condiciones donde ésta no se produce permitiría aumentar su uso industrial. Sometida a manotermosonicación, la tripsina hidroliza sustratos a temperaturas a las que dichos sustratos son resistentes al tratamiento con tripsina. La manotermosonicación acelera la hidrólisis de la fosfolipasa a 60°C. A 75°C y 90°C la fosfolipasa no es hidrolizable por la tripsina pero si el medio es sometido a manotermosonicación la tripsina sí la hidroliza. En ausencia de tripsina la fosfolipasa es siempre resistente a la manotermosonicación. Stimulation of proteases activity using manothermosonication.Proteases have many applications in the food, detergent, leather and other industries. Stimulating their activity under conditions in which this does not occur could help to increase their industrial use. Subjected to manothermosonication, trypsin hydrolyses substrates at temperatures at which said substrates are resistant to trypsin treatment. Manothermosonication accelerates phospholipase hydrolysis at 60 degrees C. At 75 degrees C and 90 degrees C, phospholipase cannot be hydrolysed by trypsin, but if the medium is subjected to manothermosonication, trypsin does hydrolyse it. Phospholipase always resists manothermosonication in the absence of trypsin.