METODO PARA LA DETERMINACION DE LA ACTIVIDAD QUITINASA BASADO EN LA UTILIZACION DE SUSTRATOS FLUORESCENTES

• Main Authors: MONFORT PRIETO ELENA, LOPEZ LLORCA LUIS VICENTE, TIKHONOV VLADIMIR, SALINAS CALVETE JESUS
• Format: Patent
• Language: Spanish
• Published: 16.09.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

Método para la determinación de la actividad quitinasa basado en la utilización de sustratos fluorescentes. El método comprende incubar una muestra de una enzima con actividad quitinasa, o de una preparación que contiene dicha enzima con actividad quitinasa, en presencia de un sustrato apropiado para dicha enzima, estando dicho sustrato marcado con un fluorocromo, bajo condiciones en las que se forman los productos de la reacción catalizada por dicha actividad quitinasa, y medir la emisión de fluorescencia. The method for determining chitinase activity based on the usage of fluorescent substrates involves the incubation of a sample of an enzyme with chitinase activity or of a preparation containing such an enzyme in the presence of an appropriate substrate. The substrate is marked with a fluorochrome, under conditions in which are formed the products of the reaction catalyzed by the chitinase activity. The emission of fluorescence is then measured.