PURIFICACION DE PEPTIDOS DE LA LECHE, ACTIVOS BIOLOGICAMENTE

• Main Authors: KUTZKO, JOSEPH, P, SHERMAN, LEE, T, HAYES, MICHAEL, L
• Format: Patent
• Language: Spanish
• Published: 01.03.2003
• Edition: 7
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FOODS OR FOODSTUFFS; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; ORGANIC CHEMISTRY; PEPTIDES; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; PROTEIN COMPOSITIONS FOR FOODSTUFFS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TRAPPING; VINEGAR; WINE; WORKING-UP PROTEINS FOR FOODSTUFFS

SE DESCRIBE UN PROCEDIMIENTO PARA SEPARAR UN COMPONENTE LACTEO SOLUBLE DE LA LECHE (POR EJEMPLO, EN UN SISTEMA DE EXTRACCION CONTINUA EN CIRCUITO CERRADO). EL PROCEDIMIENTO COMPRENDE LA UTILIZACION DE FILTRACION DE FLUJO TANGENCIAL A TRAVES DE UNA MEMBRANA PARA FORMAR UN RETENTIVO Y UN PERMEATO, SOMETIENDO EL PERMEATO A UN DISPOSITIVO CAPTOR PARA EXTRAER BASICAMENTE EL COMPONENTE LACTEO SOLUBLE, COMBINANDO EL EFLUENTE DEL DISPOSITIVO CAPTOR CON LA MUESTRA DE LECHE ORIGINAL (RETENTIVO) Y CONTINUAR ESTE PROCEDIMIENTO HASTA QUE LA LECHE SE HA PURIFICADO SUFICIENTEMENTE. PREFERIBLEMENTE, LA LECHE SE COMBINA CON UN AGENTE QUELANTE, TAL COMO EDTA, PARA MEJORAR LA EFICIENCIA DE LA PURIFICACION. ESTE PROCEDIMIENTO ES UTILIZADO VENTAJOSAMENTE CON LA LECHE PROCEDENTE DE ANIMALES TRANSGENICOS QUE HAN SIDO GENETICAMENTE ALTERADOS PARA EXPRESAR PROTEINAS EXOGENAS, TALES COMO PROTEINAS TERAPEUTICAS, EN SU LECHE. A method of separating a soluble milk component from milk is disclosed. The method involves the use of tangential flow filtration across a membrane to form a retentate and a permeate, combining the permeate with the original milk sample, and repeating this procedure until the milk has been sufficiently purified. Preferably, the milk is combined with a chelating agent, such as EDTA, to improve the purification efficiency. This procedure is advantageously employed with milk from transgenic animals which have been genetically altered to express exogenous proteins, such as therapeutic proteins, in their milk.