PROCEDIMIENTO DE ANALISIS CUANTITATIVO DEL COLESTEROL

• Main Authors: HINO, KOICHI; DAIICHI PURE ICALS CO., LTD, NAKAMURA, MITSUHIRO, MANABE, MITSUHISA
• Format: Patent
• Language: Spanish
• Published: 16.02.2001
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

UN METODO PARA DETERMINAR CUANTITATIVAMENTE COLESTEROL EN LIPOPROTEINAS DE ALTA DENSIDAD, EN EL CUAL, ANTES DE LA DETERMINACION DEL COLESTEROL POR UN METODO ENZIMATICO, SE AÑADE UN SURFACTANTE Y UNA SUBSTANCIA QUE FORMA UN COMPLEJO CON LIPOPROTEINAS DISTINTAS DE LAS LIPOPROTEINAS DE ALTA DENSIDAD A UNA MUESTRA QUE CONTIENE LIPOPROTEINAS. EL METODO NO REQUIERE NINGUN PRETRATAMIENTO COMO LA SEPARACION CENTRIFUGA. CON UNA SIMPLE OPERACION, EL COLESTEROL EN HDLS PUEDE SER MEDIDO EFECTIVAMENTE.TAMBIEN, ESTE METODO PUEDE SER ADOPTADO EN UNA VARIEDAD DE ANALIZADORES AUTOMATICOS, Y POR LO TANTO ES MUY UTIL EN EL CAMPO DE ENSAYOS CLINICOS. A method for quantitatively determining cholesterol in high density lipoproteins, in which, prior to the determination of cholesterol by an enzymatic method, a surfactant and a substance which forms a complex with lipoproteins other than high density lipoproteins are added to a sample containing lipoproteins. The method does not require any pretreatments such as centrifugal separation. With a simple operation, cholesterol in HDLs can be measured effectively. Also, this method can be adopted in a variety of automated analyzers, and thus is very useful in the field of clinical assays.