NUEVOS RECEPTORES: SU IDENTIFICACION, CARACTERIZACION, PREPARACION Y USO

• Main Authors: EVANS, RONALD MARK, HOLLENBERG, STANLEY MARK
• Format: Patent
• Language: Spanish
• Published: 16.10.1996
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; TESTING; VINEGAR; WINE

SE HA DESCUBIERTO UNA NUEVA HORMONA Y RECEPTORES SIMILARES A LA HORMONA EN LOS CUALES LAS CARACTERISTICAS DE TRANSCRIPCION Y TRANSACTIVACION ESTAN MODIFICADAS EN LOS TERMINOS DE POSICION Y / O NUMERO DE COPIA O DE OTRA MANERA EN CONTRA DEL RECEPTOR DEL PADRE, DICHOS NUEVOS RECEPTORES TIENEN INCREMENTADAS SUS PROPIEDADES DE ACTIVACION DE TRANSCRIPCION DE TRANSACTIVACION QUE SON SORPRENDENTEMENTE SUPERIORES A LAS DEL RECEPTOR PADRE. TAMBIEN SE PRESENTAN LOS METODOS RECOMBINANTES PARA PREPARAR TALES RECEPTORES Y ENSAYOS BASADOS EN EL USO DE TALES RECEPTORES PARA MONITORIZAR E IDENTIFICAR MATERIALES PUTATIVOS QUE PUEDAN AFECTAR A TALES RECEPTORES Y / O PARA LA EXPRESION POR MEDIO DE TRANSCRIPCION INDUCIDA DE UN TESTIGO U OTRO DESEADO, PREFERIBLEMENTE UN GEN HETEROLOGO O PRODUCTO DE DNA. Disclosed are novel hormone and hormone-like receptors wherein the trans-activation transcription domain(s) is modified in terms of position and/or copy number or otherwise versus parent receptor, such novel receptors having increased trans-activation transcription activation properties surprisingly superior to the parent receptor. Also disclosed are the recombinant methods and means for preparing such receptors and assays based upon the use of such receptors for screening and identifying putative materials that can affect such receptors and/or for the expression via induced transcription of a reporter or other desired, preferably heterologous gene or DNA product.