Molecular diagnostic methods for the detection of Brucella abortus and differentiation from vaccine strain B19

• Main Authors: SANGARI GARCIA FELIX JAVIER, AGUERO BALBIN JESUS, GARCIA LOBO JUAN MARIA
• Format: Patent
• Language: English; Spanish
• Published: 01.12.1995
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Molecular diagnostic methods for the detection of Brucella abortus and differentiation from vaccine strain B19. A series of molecular diagnostic methods which can be used to differentiate between the Brucella abortus B19 vaccine lineage and all other lineages of the genus Brucella are described. In one case, amplification of the genome is applied using oligonucleotides derived from the region containing the genes coding for the erythritol utilization route as indicators. Another group of methods is based on the hybridization of genome DNA using test oligonucleotides from the eri region mentioned. In this case the hybridization technique known as the Southern type or alternatively the "dot blot" procedure may be used. Finally these methods are applied to the identification of micro-organisms of the genus Brucella in any biological sample. The new procedures have advantages over conventional immunological and culture methods providing greater specificity and speed. METODOS DE DIAGNOSTICO MOLECULAR PARA LA DETECCION DE CVABRUCELLA ABORTUS Y SU DIFERENCIACION DE LA CEPA VACUNAL B19. SE DESCRIBEN UNA SERIE DE METODOS DE DIAGNOSTICO MOLECULAR QUE PERMITEN A DIFERENCIACION ENTRE LA ESTIRPE VACUNAL CVABRUCELLA ABORTUS B19 Y TODAS LAS DEMAS ESTIRPES DEL GENERO CVABRUCELLA. EN UN CASO SE APLICA LA AMPLIFICACION DE GENOMA, USANDO COMO INICIADORES OLIGONUCLEOTIDOS DERIVADOS DE LA REGION QUE CONTIENE LOS GENES CODIFICANTES DE LA RUTA DE UTILIZACION DEL ERITRITOL. OTRO GRUPO DE METODOS SE BASA EN LA HIBRIDACION DE ADN GENOMICO USANDO COMO SONDAS OLIGONUCLEOTIDOS DE LA MENCIONADA REGION CVAERI. EN ESTE CASO PUEDEN APLICARSE SEGUN LA TECNICA DENOMINADA "HIBRIDACION TIPO SOUTHERN" O ALTERNATIVAMENTE POR EL PROCEDIMIENTO DEL "DOT BLOT". FINALMENTE SE APLICAN ESTOS METODOS PARA LA IDENTIFICACION DE GERMENES DEL GENERO CVABRUCELLA EN CUALQUIER MUESTRA BIOLOGICA. LOS NUEVOS PROCEDIMIENTOS SON VENTAJOSOS SOBRE LOS METODOS TRADICIONALES, INMUNOLOGICOS O DE CULTIVO, APORTANDO MAYOR ESPECIFICIDAD Y RAPIDEZ.