Microbial process for preparing mevinolin by a strain of aspergillus

• Main Authors: NAGY KAROLY, ILKOY EVA, SZABO ISTVAN MIHALY, AMBRUS GABOR, CSEKE LASZLO, MAGYI ISTVAN, SZABO ISTVAN, MORAVCSIK IMRE, ANDOR ATTILA, VARGA ILONA, KASZAS MIHALY, KISS LAJOS, POLYA KALMAN, ERDEI JANOS, HALASZ EDIT, JEKKEL ANTONIA, KISS ANDRAS
• Format: Patent
• Language: English; Spanish
• Published: 16.01.1995
• Edition: 5
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HETEROCYCLIC COMPOUNDS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; SPIRITS; VINEGAR; WINE

The invention refers to a new microbial process for preparing beta , delta -dihydroxy-7-[1,2,6,7,8,8a-hexahydro-2.6-dimethyl-8-(2-methyl-butyryloxy)-naphthalen-1-yl]-heptanoic acid delta -lactone of formula (I) and beta , delta -dihydroxy-7-[1,2,6,7,8,8a-hexahydro-2.6-dimethyl-8-(2-methylbutyryloxy)-naphtalen-1-yl]-heptanoic acid of formula (II), < IMAGE > (I) < IMAGE > (II) by the aerobic fermentation of the submerged culture of an imperfect fungus strain biosynthesizing the above compounds, in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the product of formula (I), which comprises cultivating a strain of the new Aspergillus obscurus fungus species producing the above compound(s) of formulas (I) and/or (II), in a temperature range of 25 DEG to 30 DEG C. and, if desired, separating the product(s) formed from the fermentation broth, then isolating it in the lactone form of formula (I) and, if desired, purifying the same. EL INVENTO SE REFIERE A UN PROCESO MICROBIANO PARA LA PREPARACION DE LA DELTA-LACTONA DEL ACIDO BETA, DELTADIHIDROXI-7-[1,2,6,7,8,8A-HEXAHIDRO-2,6-DIMETIL-8- (2METILBUTIRILOXI) -NAFTALEN-1-IL]-EPTANOICO DE FORMULA (I) Y DEL ACIDO BETA, DELTA-DIHIDROXI-7-[1,2,6,7,8A-HEXAHIDRO- 2,6DIMETIL-8- (2-METILBUTIRILOXI) -NAFTALEN-1-IL]-HEPTANCICO DE FORMULA (II), POR LA FERMENTACION AEROBICA DEL CULTIVO SUMERGIDO DE UNA CEPA DE HONGOS IMPERFECTA, BIOSINTETIZANDO LOS COMPUESTOS ARRIBA INDICADOS EN UN MEDIO NUTRIENTE QUE CONTIENE FUENTES DE CARBONO Y NITROGENO APROVECHABLES ASI COMO SALES MINERALES, Y AISLANDO EL PRODUCTO DE FORMULA (I) QUE COMPRENDE EL CULTIVO DE UNA CEPA DE UNA ESPECIE DE HONGOS NUEVA ASPERGILLUS OBSCURUS QUE PRODUCE EL/LOS COMPUESTO(S) ARRIBA INDICADOS DE FORMULAS (I) Y/O (II) DENTRO DE UN RANGO DE TEMPERATURAS DE 25 A 30GC Y, SI SE DESEA, SEPARANDO EL/LOS PRODUCTO(S) FORMADO EN EL CALDO DE FERMENTACION, AISLANDOLO DESPUES EN SU FORMA DE LACTONA DE FORMULA (I) Y, SI SE DESEA, PURIFICANDO EL MISMO.