IMMUNOASSAY METHOD AND KIT

• Main Authors: ROSSI DEBRA J, KUEHN CHRISTA G, ISIED STEPHEN S
• Format: Patent
• Language: English; Spanish
• Published: 16.04.1988
• Edition: 4
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

A method of binding an antibody with protein A cells is provided which includes a sequence of incubation and dilution steps to produce a preselected amount and concentration of antibody with a preselected distribution of antibody among the protein A cells. In addition, a method is provided for preparing an antibody entrapped porous matrix and apparatus which includes a specific porous matrix with a preselected position of antibody bound bacterium cells therein along with means for drawing fluids through the porous medium and means for facilitating the deposition of fluids onto the surface of the porous matrix. The apparatus and method is useful for testing for the level of progesterone in animal body fluids, such as milk, plasma, serum, whole blood and saliva. SE PROPORCIONA UN METODO PARA UNIR UN ANTICUERPO CON CELULAS DE PROTEINA A QUE INCLUYE UNA SECUENCIA DE ETAPAS DE INCUBACION Y DILUCION PARA PRODUCIR UNA CANTIDAD Y UNA CONCENTRACION PRESELECCIONADAS DE ANTICUERPO CON UNA DISTRIBUCION PRESELECCIONADA DE ANTICUERPO ENTRE LAS CELULAS DE PROTEINA A. ADEMAS, SE PROPORCIONA UN METODO PARA PREPARAR UNA MATRIZ POROSA CON ANTICUERPO RETENIDO Y UN APARATO QUE INCLUYE UNA MATRIZ POROSA ESPECIFICA CON UNA POSICION PRESELECCIONADA DE CELULAS BACTERIANAS UNIDAS A ANTICUERPO EN ELLA JUNTO CON MEDIOS PARA ARRASTRAR FLUIDOS A TRAVES DEL MEDIO POROSO Y MEDIOS PARA FACILITAR LA DEPOSICION DE FLUIDOS SOBRE LA SUPERFICIE DE LA MATRIZ POROSA. EL APARATO Y EL METODO SON UTILES PARA ENSAYAR EL NIVEL DE PROGESTERONA EN FLUIDOS CORPORALES DE ANIMALES, TALES COMO LECHE, PLASMA, SUERO, SANGRE TOTAL Y SALIVA.