ENGINEERED ACR PROTEINS FOR MODULATING CRISPR ACTIVITY

• Main Authors: SHELLE, Michael, RABUKA, David, DE TACCA, Luisa Mayumi Arake, SHARRAR, Allison
• Format: Patent
• Language: English; French; German
• Published: 23.04.2025
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided are compositions and methods that include an engineered anti-CRISPR (Acr) protein that can achieve a balance in which Cas proteins retain activity to perform on-target nucleic acid targeting functions (e.g., DNA cleavage for gene editing applications), but are inhibited by a subject Acr pro...