ENGINEERED ACR PROTEINS FOR MODULATING CRISPR ACTIVITY

Provided are compositions and methods that include an engineered anti-CRISPR (Acr) protein that can achieve a balance in which Cas proteins retain activity to perform on-target nucleic acid targeting functions (e.g., DNA cleavage for gene editing applications), but are inhibited by a subject Acr pro...

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Bibliographic Details
Main Authors SHELLE, Michael, RABUKA, David, DE TACCA, Luisa Mayumi Arake, SHARRAR, Allison
Format Patent
LanguageEnglish
French
German
Published 23.04.2025
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Provided are compositions and methods that include an engineered anti-CRISPR (Acr) protein that can achieve a balance in which Cas proteins retain activity to perform on-target nucleic acid targeting functions (e.g., DNA cleavage for gene editing applications), but are inhibited by a subject Acr protein to a degree that decreases off-target activity - thus resulting in an increased ratio of on-target to off-target nucleic acid targeting events. In some cases, a subject composition (e.g., a subject system) includes a CRISPR nuclease or a nucleic acid encoding it. Methods of using a subject Acr polypeptide together with a CRISPR nuclease to achieve target nucleic acid modification are provided.
Bibliography:Application Number: EP20230824718